FastQCFastQC Report
Thu 4 Oct 2018
B7_H3K4me1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB7_H3K4me1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41103741
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65050.040.8337631
CTCGTAT48300.040.14571842
TCGTATG50200.039.7451443
TCTCGTA51450.037.87672841
CGTATGC52750.037.70821844
ATCGGAA72100.037.1273652
TATGCCG56250.036.3204446
AATCTCG57300.035.62228439
ATGCCGT57400.033.7397547
CGTCTGA81500.032.7172916
ACACGTC83500.032.057713
CACGACG21300.030.9721915
ACGACGC21050.030.35549216
ATCTCGT67550.029.25098640
ACGTCTG91050.029.2477615
TCGCGTA5000.029.07560341
TGCCGTC62500.029.06054148
GCCGTCT63750.027.4197749
CGACGCT23450.026.95413417
TCGGAAG100700.026.7885533
Produced by FastQC (version 0.11.5)