FastQCFastQC Report
Thu 4 Oct 2018
B7_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameB7_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42117831
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN487070.11564460667502084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGC39750.09.20487426
ACGTTTT484950.09.18084770
TAGTGAT483300.08.682754570
CGAATCG7152.2964741E-88.6018974
CTCGAAC91800.08.52320814
CGCCACC77400.08.37494370
TCGAACT93900.08.33259215
ATCCGCC44600.08.20605427
AGTCGCG5051.072029E-48.1220441
CGACGTG14156.190021E-98.03695570
TTTTCCG454400.07.94024236
CGAACTC92200.07.930362716
ACGTATT76800.07.922104470
TTTCCGT463050.07.81313137
TTCCGTG459950.07.73209528
TCACGTC463250.07.51829131
CACGACG10052.2791937E-97.479401615
AGACGCG10557.6761353E-107.4516221
TCCTCGC454650.07.41675718
ACGACGC9252.9516741E-87.387517516
Produced by FastQC (version 0.11.5)