[2016-04-07 16:52:11] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2016-04-07 16:52:11] Checking for Bowtie Bowtie version: 2.2.5.0 [2016-04-07 16:52:11] Checking for Bowtie index files (genome).. [2016-04-07 16:52:11] Checking for reference FASTA file [2016-04-07 16:52:11] Generating SAM header for ../anno/genome [2016-04-07 16:52:36] Reading known junctions from GTF file [2016-04-07 16:52:43] Preparing reads left reads: min. length=50, max. length=50, 28864969 kept reads (25706 discarded) right reads: min. length=50, max. length=50, 28855610 kept reads (35065 discarded) [2016-04-07 17:00:52] Building transcriptome data files A3CO_13059_CGATGT/tmp/genes [2016-04-07 17:01:22] Building Bowtie index from genes.fa [2016-04-07 17:03:46] Mapping left_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:14:24] Mapping right_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:25:25] Resuming TopHat pipeline with unmapped reads [2016-04-07 17:25:25] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:29:51] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:30:47] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:31:42] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:35:46] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:36:40] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:37:33] Searching for junctions via segment mapping [2016-04-07 17:51:50] Retrieving sequences for splices [2016-04-07 17:53:24] Indexing splices [2016-04-07 17:58:54] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:00:01] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:00:59] Joining segment hits [2016-04-07 18:02:40] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:03:40] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:04:38] Joining segment hits [2016-04-07 18:06:20] Reporting output tracks ----------------------------------------------- [2016-04-07 18:26:42] A summary of the alignment counts can be found in A3CO_13059_CGATGT/align_summary.txt [2016-04-07 18:26:42] Run complete: 01:34:31 elapsed