[2016-04-07 16:52:41] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2016-04-07 16:52:41] Checking for Bowtie Bowtie version: 2.2.5.0 [2016-04-07 16:52:41] Checking for Bowtie index files (genome).. [2016-04-07 16:52:41] Checking for reference FASTA file [2016-04-07 16:52:41] Generating SAM header for ../anno/genome [2016-04-07 16:53:00] Reading known junctions from GTF file [2016-04-07 16:53:06] Preparing reads left reads: min. length=50, max. length=50, 35428086 kept reads (31902 discarded) right reads: min. length=50, max. length=50, 35417903 kept reads (42085 discarded) [2016-04-07 17:03:14] Building transcriptome data files A3DE_13060_TGACCA/tmp/genes [2016-04-07 17:03:30] Building Bowtie index from genes.fa [2016-04-07 17:05:54] Mapping left_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:20:47] Mapping right_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:35:46] Resuming TopHat pipeline with unmapped reads [2016-04-07 17:35:46] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:41:11] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:42:11] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:43:03] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:47:51] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:48:48] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:49:40] Searching for junctions via segment mapping [2016-04-07 18:06:23] Retrieving sequences for splices [2016-04-07 18:08:06] Indexing splices [2016-04-07 18:15:12] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:16:38] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:17:42] Joining segment hits [2016-04-07 18:19:33] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:20:36] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:21:37] Joining segment hits [2016-04-07 18:23:26] Reporting output tracks ----------------------------------------------- [2016-04-07 18:50:08] A summary of the alignment counts can be found in A3DE_13060_TGACCA/align_summary.txt [2016-04-07 18:50:08] Run complete: 01:57:27 elapsed