[2016-04-07 16:52:40] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2016-04-07 16:52:40] Checking for Bowtie Bowtie version: 2.2.5.0 [2016-04-07 16:52:40] Checking for Bowtie index files (genome).. [2016-04-07 16:52:40] Checking for reference FASTA file [2016-04-07 16:52:40] Generating SAM header for ../anno/genome [2016-04-07 16:52:48] Reading known junctions from GTF file [2016-04-07 16:52:55] Preparing reads left reads: min. length=50, max. length=50, 34296765 kept reads (30334 discarded) right reads: min. length=50, max. length=50, 34287739 kept reads (39360 discarded) [2016-04-07 17:03:21] Building transcriptome data files A6DE_13062_CCGTCC/tmp/genes [2016-04-07 17:03:38] Building Bowtie index from genes.fa [2016-04-07 17:05:48] Mapping left_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:19:32] Mapping right_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:33:15] Resuming TopHat pipeline with unmapped reads [2016-04-07 17:33:16] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:38:12] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:39:04] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:39:53] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:44:33] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:45:37] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:46:40] Searching for junctions via segment mapping [2016-04-07 18:08:04] Retrieving sequences for splices [2016-04-07 18:09:48] Indexing splices [2016-04-07 18:19:13] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:20:41] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:22:03] Joining segment hits [2016-04-07 18:24:29] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:26:02] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:27:46] Joining segment hits [2016-04-07 18:30:09] Reporting output tracks ----------------------------------------------- [2016-04-07 18:56:21] A summary of the alignment counts can be found in A6DE_13062_CCGTCC/align_summary.txt [2016-04-07 18:56:21] Run complete: 02:03:41 elapsed