[2016-04-07 16:53:09] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2016-04-07 16:53:09] Checking for Bowtie Bowtie version: 2.2.5.0 [2016-04-07 16:53:10] Checking for Bowtie index files (genome).. [2016-04-07 16:53:10] Checking for reference FASTA file [2016-04-07 16:53:10] Generating SAM header for ../anno/genome [2016-04-07 16:53:20] Reading known junctions from GTF file [2016-04-07 16:53:27] Preparing reads left reads: min. length=50, max. length=50, 33294650 kept reads (30187 discarded) right reads: min. length=50, max. length=50, 33284714 kept reads (40123 discarded) [2016-04-07 17:03:04] Building transcriptome data files A7DE_13064_GATCAG/tmp/genes [2016-04-07 17:03:23] Building Bowtie index from genes.fa [2016-04-07 17:05:33] Mapping left_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:18:25] Mapping right_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:31:40] Resuming TopHat pipeline with unmapped reads [2016-04-07 17:31:41] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:36:07] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:36:58] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:37:45] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:41:59] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:42:51] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:43:42] Searching for junctions via segment mapping [2016-04-07 18:00:58] Retrieving sequences for splices [2016-04-07 18:02:41] Indexing splices [2016-04-07 18:12:02] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:13:39] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:14:59] Joining segment hits [2016-04-07 18:17:16] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:18:42] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:20:07] Joining segment hits [2016-04-07 18:22:24] Reporting output tracks ----------------------------------------------- [2016-04-07 18:47:22] A summary of the alignment counts can be found in A7DE_13064_GATCAG/align_summary.txt [2016-04-07 18:47:22] Run complete: 01:54:12 elapsed