[2016-04-07 16:54:11] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2016-04-07 16:54:11] Checking for Bowtie Bowtie version: 2.2.5.0 [2016-04-07 16:54:11] Checking for Bowtie index files (genome).. [2016-04-07 16:54:11] Checking for reference FASTA file [2016-04-07 16:54:11] Generating SAM header for ../anno/genome [2016-04-07 16:54:21] Reading known junctions from GTF file [2016-04-07 16:54:28] Preparing reads left reads: min. length=50, max. length=50, 33854791 kept reads (30306 discarded) right reads: min. length=50, max. length=50, 33844866 kept reads (40231 discarded) [2016-04-07 17:04:09] Building transcriptome data files A8CO_13065_CGTACG/tmp/genes [2016-04-07 17:04:27] Building Bowtie index from genes.fa [2016-04-07 17:06:51] Mapping left_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:21:30] Mapping right_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:36:39] Resuming TopHat pipeline with unmapped reads [2016-04-07 17:36:40] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:40:31] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:41:17] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:41:58] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:45:28] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:46:13] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:46:58] Searching for junctions via segment mapping [2016-04-07 18:01:23] Retrieving sequences for splices [2016-04-07 18:02:56] Indexing splices [2016-04-07 18:07:51] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:08:45] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:09:29] Joining segment hits [2016-04-07 18:11:10] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:11:59] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:12:47] Joining segment hits [2016-04-07 18:14:27] Reporting output tracks ----------------------------------------------- [2016-04-07 18:39:29] A summary of the alignment counts can be found in A8CO_13065_CGTACG/align_summary.txt [2016-04-07 18:39:29] Run complete: 01:45:18 elapsed