[2016-04-07 16:54:47] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2016-04-07 16:54:47] Checking for Bowtie Bowtie version: 2.2.5.0 [2016-04-07 16:54:47] Checking for Bowtie index files (genome).. [2016-04-07 16:54:47] Checking for reference FASTA file [2016-04-07 16:54:47] Generating SAM header for ../anno/genome [2016-04-07 16:54:58] Reading known junctions from GTF file [2016-04-07 16:55:04] Preparing reads left reads: min. length=50, max. length=50, 33489937 kept reads (29611 discarded) right reads: min. length=50, max. length=50, 33480762 kept reads (38786 discarded) [2016-04-07 17:04:45] Building transcriptome data files A8DE_13066_ATTCCT/tmp/genes [2016-04-07 17:05:03] Building Bowtie index from genes.fa [2016-04-07 17:07:26] Mapping left_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:21:35] Mapping right_kept_reads to transcriptome genes with Bowtie2 [2016-04-07 17:35:42] Resuming TopHat pipeline with unmapped reads [2016-04-07 17:35:42] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:40:31] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:41:13] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:41:53] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2016-04-07 17:46:08] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2016-04-07 17:46:55] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2016-04-07 17:47:39] Searching for junctions via segment mapping [2016-04-07 18:03:13] Retrieving sequences for splices [2016-04-07 18:04:50] Indexing splices [2016-04-07 18:11:36] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:12:35] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:13:28] Joining segment hits [2016-04-07 18:15:14] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2016-04-07 18:16:11] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2016-04-07 18:17:07] Joining segment hits [2016-04-07 18:18:55] Reporting output tracks ----------------------------------------------- [2016-04-07 18:43:56] A summary of the alignment counts can be found in A8DE_13066_ATTCCT/align_summary.txt [2016-04-07 18:43:56] Run complete: 01:49:09 elapsed