##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	6494_GGCTAC_R1.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	131737035	
Filtered Sequences	0	
Sequence length	100	
%GC	47	
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.167675012573344	31.0	28.0	33.0	25.0	34.0
2	31.741793459978812	33.0	31.0	33.0	30.0	34.0
3	32.02198558666513	33.0	31.0	33.0	30.0	34.0
4	36.05271075062529	37.0	35.0	37.0	35.0	37.0
5	36.09562797583838	37.0	35.0	37.0	35.0	37.0
6	36.119580473327034	37.0	36.0	37.0	35.0	37.0
7	36.12927029973007	37.0	36.0	37.0	35.0	37.0
8	36.10930582277034	37.0	37.0	37.0	35.0	37.0
9	37.917470876735614	39.0	38.0	39.0	35.0	39.0
10-14	37.99501089272277	39.4	37.8	39.4	35.2	39.4
15-19	39.18411658953763	40.8	39.0	41.0	36.0	41.0
20-24	39.21502808075193	41.0	39.0	41.0	36.0	41.0
25-29	38.97324359547033	40.2	39.0	41.0	35.2	41.0
30-34	38.73186758150432	40.0	38.0	41.0	35.0	41.0
35-39	38.53604387862532	40.0	38.0	41.0	34.2	41.0
40-44	38.409104344879175	40.0	38.0	41.0	34.0	41.0
45-49	38.17709125607693	40.0	37.8	41.0	33.6	41.0
50-54	37.73615897306327	40.0	36.8	41.0	33.0	41.0
55-59	37.18714277272143	39.0	35.8	40.8	32.0	41.0
60-64	36.39695256387089	38.2	35.0	40.0	30.8	41.0
65-69	35.410424485415206	36.8	34.2	39.6	29.8	40.8
70-74	34.37766030030963	35.6	33.8	38.4	28.8	40.0
75-79	32.637375568684995	34.2	31.8	36.0	26.8	38.6
80-84	32.62351977938474	34.6	32.6	35.6	27.2	37.2
85-89	31.665480927212307	34.0	31.6	35.0	25.6	36.2
90-94	30.320905341463014	34.0	31.0	35.0	18.6	35.0
95-99	18.972820537520068	19.0	6.6	33.4	2.0	35.0
100	15.121274712156684	17.0	2.0	28.0	2.0	31.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	182.0
3	465.0
4	814.0
5	2178.0
6	4768.0
7	12194.0
8	28009.0
9	46298.0
10	66840.0
11	89293.0
12	109207.0
13	130458.0
14	153393.0
15	180325.0
16	212156.0
17	247649.0
18	285456.0
19	321279.0
20	359638.0
21	407500.0
22	475226.0
23	581668.0
24	715983.0
25	862463.0
26	1064749.0
27	1344155.0
28	1672631.0
29	2109075.0
30	2691597.0
31	3516725.0
32	4833968.0
33	7041710.0
34	1.1082952E7
35	1.8310712E7
36	2.6684346E7
37	2.8235946E7
38	1.6636352E7
39	1218628.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.952406574072675	10.068855693105458	4.856443163670819	32.12229456915105
2	26.026753980002663	11.201708767773619	33.304494062736424	29.467043189487303
3	23.47926913642773	14.920570362009439	24.244828343069965	37.355332158492864
4	25.900302067675955	23.054265643674157	22.952742180663165	28.09269010798672
5	27.725653609859975	26.66807781122446	24.272062142585796	21.33420643632977
6	23.23295723180653	29.493924772179668	24.641366795601556	22.631751200412246
7	17.41151529636294	23.050767007166968	41.44181095316135	18.095906743308742
8	19.28076261925889	23.11539423974435	33.5054906921201	24.09835244887666
9	19.48210007914631	21.41052058747185	35.28174973727016	23.82562959611168
10-14	22.696174317232806	27.0114078677693	27.9941209816823	22.298296833315593
15-19	22.939523100213034	25.34731736277819	28.329615221679138	23.383544315329637
20-24	22.77629369751642	25.566920190666202	28.22811580661429	23.428670305203088
25-29	22.715687435631374	25.609143979809097	28.19463221776711	23.480536366792414
30-34	22.732781342938306	25.560028094875126	27.999203515814358	23.707987046372214
35-39	22.666132341329938	25.578411139450512	28.09640714337252	23.65904937584703
40-44	22.69686250578297	25.562002038331805	28.077560294141286	23.66357516174394
45-49	22.717561905031193	25.483675530764327	28.053805987151726	23.744956577052754
50-54	22.617970889017332	25.5254921518208	28.114410013499082	23.742126945662786
55-59	22.64389539357706	25.462459968072	28.111379005911285	23.782265632439657
60-64	22.63265719436645	25.411247858982872	28.04999040401735	23.906104542633326
65-69	22.663571825683267	25.45854863584894	28.161708368811432	23.716171169656366
70-74	22.834690686674623	25.582261679257467	27.891488925547094	23.691558708520816
75-79	22.849061387138214	25.50608127447958	27.805603713408576	23.839253624973626
80-84	22.95626096754005	25.442301219653828	27.829947668360973	23.771490144445153
85-89	23.08162388927176	25.63552597771282	27.515115342008745	23.767734791006678
90-94	23.299986852383448	25.576016381359096	27.277339019048018	23.846657747209434
95-99	23.717436780427036	25.663455891989916	27.081135091506614	23.53797223607643
100	25.69851067317554	25.64355725783566	26.754566018583915	21.903366050404884
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	85.07470114322372
2	55.493797169489966
3	60.834601294920596
4	53.99299217566268
5	49.05986004618975
6	45.86470843221878
7	35.50742203967168
8	43.37911506813555
9	43.30772967525799
10-14	44.9944711505484
15-19	46.32306741554267
20-24	46.20496400271951
25-29	46.196223802423795
30-34	46.44076838931051
35-39	46.32518171717697
40-44	46.36043766752691
45-49	46.46251848208395
50-54	46.36009783468012
55-59	46.426161026016715
60-64	46.53876173699978
65-69	46.379742995339626
70-74	46.52624939519544
75-79	46.68831501211184
80-84	46.7277511119852
85-89	46.849358680278435
90-94	47.14664459959288
95-99	47.25540901650347
100	47.601876723580425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	508.0
1	331.5
2	142.5
3	143.5
4	161.5
5	178.5
6	227.0
7	263.0
8	332.5
9	460.0
10	611.5
11	798.0
12	1058.5
13	1440.5
14	2098.5
15	3302.5
16	5373.5
17	8588.5
18	13506.5
19	22519.5
20	36615.0
21	58244.0
22	91546.0
23	134287.0
24	193215.5
25	273013.0
26	381479.5
27	524884.5
28	696012.0
29	887140.5
30	1102600.5
31	1348687.5
32	1606197.5
33	1870229.0
34	2181018.0
35	2516988.0
36	2851581.5
37	3238840.0
38	3641564.0
39	4007553.0
40	4358594.0
41	4690141.0
42	5007184.5
43	5256488.0
44	5398673.5
45	5522229.5
46	5636615.0
47	5569220.5
48	5421809.5
49	5279154.0
50	5123446.5
51	4959093.0
52	4647150.0
53	4332432.0
54	4065308.5
55	3741863.0
56	3363836.5
57	2935705.5
58	2591993.5
59	2340752.5
60	2072210.0
61	1834221.0
62	1604931.0
63	1396910.5
64	1237825.5
65	1078566.0
66	936771.0
67	813499.0
68	681931.5
69	538590.5
70	407490.0
71	306772.5
72	233690.0
73	182321.5
74	135861.0
75	95470.5
76	73545.5
77	57043.5
78	40380.0
79	25584.0
80	14489.5
81	8636.0
82	5236.0
83	3178.0
84	2059.0
85	1521.0
86	1137.5
87	754.0
88	438.0
89	279.5
90	191.0
91	106.0
92	54.0
93	36.0
94	24.0
95	18.0
96	12.5
97	11.0
98	14.5
99	21.5
100	19.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.03081138117310747
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.003052596409202621
15-19	0.01490469251869833
20-24	0.0
25-29	0.0012608451374361053
30-34	0.018360516463726394
35-39	5.055525957450006E-5
40-44	2.1907279148950028E-4
45-49	0.0028725407399673142
50-54	8.277095351356587E-4
55-59	0.0
60-64	0.07011073233885975
65-69	0.13417578435707164
70-74	0.19756388171329348
75-79	0.14178351592625413
80-84	0.055453350684566416
85-89	0.16451167281850546
90-94	0.21062140953756855
95-99	10.249361085134488
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.31737035E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	87.60533555503486
#Duplication Level	Relative count
1	100.0
2	54.19409046352052
3	35.429211595014706
4	26.249824954488165
5	21.642627083041592
6	17.900154040050413
7	15.715586052373617
8	13.541520795406806
9	12.771320543341268
10++	402.7377118050693
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	41084735	3.3300562	4.409197	9
TCTTC	42831025	2.8338983	5.9311852	7
CCAGG	24556130	2.602913	5.9029937	2
TTCTT	45564045	2.5800562	5.011365	6
CTTCA	33930060	2.48821	5.7084527	6
CTCCA	28788355	2.4668236	8.364095	1
CCAGC	23195945	2.3896372	5.8898244	2
CAGCT	27099850	2.389285	5.535928	3
TCCAG	26764945	2.359758	6.7397556	2
GGCAG	21186040	2.3106241	11.347821	1
CAGGA	22940785	2.3065653	5.1073995	3
CTCCT	29393495	2.2724578	5.8940825	1
CTGCT	27457950	2.1842012	5.4597697	3
GCAGG	19730160	2.1518407	5.097575	2
GCCAG	20182250	2.1392882	11.78663	1
GGCTG	21574035	2.1229231	8.415544	1
GCTGG	21365105	2.102364	6.440885	1
CCCAG	20121255	2.0728838	6.9681864	1
CCACA	21781150	2.06861	5.5726776	2
CCTGG	20152840	1.9273447	5.1297736	1
GGCCA	17718620	1.8781471	8.062006	1
GGGCA	16828870	1.8354161	7.0198064	1
GGGGG	14962795	1.8213533	9.535551	1
CTCTG	22885265	1.8204567	5.1649933	1
CCAGA	18571710	1.8148032	5.4994316	2
GGGAG	16107430	1.8075305	6.7470956	1
GGGGC	14181965	1.6777917	6.882024	1
GCTCC	17765260	1.6512576	5.455988	1
GGGGA	14666750	1.6458615	7.157799	1
GCCAC	15708705	1.6183046	8.099069	1
CCCCA	15742710	1.5762299	5.640662	1
GCCTG	15910400	1.5216131	7.134165	1
GGCCT	15606395	1.4925389	5.4717517	1
GCCTC	15972420	1.4846156	6.209764	1
GTGGG	14455640	1.4635923	6.394421	1
GGGGT	13968700	1.4142909	7.0125785	1
GTCCA	16003885	1.4109983	9.290385	1
GCCCA	13569275	1.3979014	7.1524243	1
GCCAA	14127335	1.3805047	5.572314	1
GGCAT	14935040	1.3548375	5.54831	1
GTCTG	16460980	1.3472865	6.1431975	1
GCCTT	16855925	1.3408402	5.2929497	1
GGCAA	13097980	1.3169273	5.3036137	1
GCCAT	14838665	1.3082654	5.7831726	1
GTCAG	14006695	1.2706226	6.8097825	1
GTCCT	15289750	1.2162557	7.1161427	1
GCCCC	10822905	1.175455	5.378905	1
GCCCT	11604615	1.0786338	5.526143	1
GTCCC	10414100	0.967977	5.217869	1
>>END_MODULE