[2024-04-23 12:32:18] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2024-04-23 12:32:18] Checking for Bowtie Bowtie 2 not found, checking for older version.. Bowtie version: 1.1.1.0 [2024-04-23 12:32:18] Checking for Bowtie index files (genome).. [2024-04-23 12:32:18] Checking for reference FASTA file [2024-04-23 12:32:18] Generating SAM header for /no_backup/ldicroce/eblanco/molbio/indexes/mm10/genome [2024-04-23 12:32:20] Reading known junctions from GTF file [2024-04-23 12:32:24] Preparing reads left reads: min. length=50, max. length=50, 45478961 kept reads (566 discarded) [2024-04-23 12:38:34] Building transcriptome data files /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_114K8_08597/tmp/refGene [2024-04-23 12:38:48] Building Bowtie index from refGene.fa [2024-04-23 12:50:18] Mapping left_kept_reads to transcriptome refGene with Bowtie [2024-04-23 13:22:23] Resuming TopHat pipeline with unmapped reads [2024-04-23 13:22:23] Mapping left_kept_reads.m2g_um to genome genome with Bowtie [2024-04-23 13:25:48] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie (1/2) [2024-04-23 13:30:10] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie (2/2) [2024-04-23 13:34:22] Searching for junctions via segment mapping [2024-04-23 13:36:42] Retrieving sequences for splices [2024-04-23 13:38:13] Indexing splices [2024-04-23 13:38:27] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie (1/2) [2024-04-23 13:39:04] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie (2/2) [2024-04-23 13:39:41] Joining segment hits [2024-04-23 13:41:22] Reporting output tracks ----------------------------------------------- [2024-04-23 13:54:38] A summary of the alignment counts can be found in /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_114K8_08597/align_summary.txt [2024-04-23 13:54:38] Run complete: 01:22:20 elapsed