[2024-04-23 12:32:17] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2024-04-23 12:32:17] Checking for Bowtie Bowtie 2 not found, checking for older version.. Bowtie version: 1.1.1.0 [2024-04-23 12:32:17] Checking for Bowtie index files (genome).. [2024-04-23 12:32:17] Checking for reference FASTA file [2024-04-23 12:32:17] Generating SAM header for /no_backup/ldicroce/eblanco/molbio/indexes/mm10/genome [2024-04-23 12:32:19] Reading known junctions from GTF file [2024-04-23 12:32:23] Preparing reads left reads: min. length=50, max. length=50, 46092942 kept reads (361 discarded) [2024-04-23 12:38:24] Building transcriptome data files /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_114W1_08593/tmp/refGene [2024-04-23 12:38:39] Building Bowtie index from refGene.fa [2024-04-23 12:50:35] Mapping left_kept_reads to transcriptome refGene with Bowtie [2024-04-23 13:20:09] Resuming TopHat pipeline with unmapped reads [2024-04-23 13:20:09] Mapping left_kept_reads.m2g_um to genome genome with Bowtie [2024-04-23 13:23:20] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie (1/2) [2024-04-23 13:26:39] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie (2/2) [2024-04-23 13:29:55] Searching for junctions via segment mapping [2024-04-23 13:32:10] Retrieving sequences for splices [2024-04-23 13:33:39] Indexing splices [2024-04-23 13:33:54] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie (1/2) [2024-04-23 13:34:25] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie (2/2) [2024-04-23 13:34:55] Joining segment hits [2024-04-23 13:36:25] Reporting output tracks ----------------------------------------------- [2024-04-23 13:49:40] A summary of the alignment counts can be found in /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_114W1_08593/align_summary.txt [2024-04-23 13:49:40] Run complete: 01:17:22 elapsed