[2024-04-23 12:32:17] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2024-04-23 12:32:17] Checking for Bowtie Bowtie 2 not found, checking for older version.. Bowtie version: 1.1.1.0 [2024-04-23 12:32:17] Checking for Bowtie index files (genome).. [2024-04-23 12:32:17] Checking for reference FASTA file [2024-04-23 12:32:17] Generating SAM header for /no_backup/ldicroce/eblanco/molbio/indexes/mm10/genome [2024-04-23 12:32:19] Reading known junctions from GTF file [2024-04-23 12:32:23] Preparing reads left reads: min. length=50, max. length=50, 46319406 kept reads (215 discarded) [2024-04-23 12:39:26] Building transcriptome data files /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_114W3_08594/tmp/refGene [2024-04-23 12:39:38] Building Bowtie index from refGene.fa [2024-04-23 12:50:34] Mapping left_kept_reads to transcriptome refGene with Bowtie [2024-04-23 13:25:52] Resuming TopHat pipeline with unmapped reads [2024-04-23 13:25:52] Mapping left_kept_reads.m2g_um to genome genome with Bowtie [2024-04-23 13:29:47] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie (1/2) [2024-04-23 13:33:53] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie (2/2) [2024-04-23 13:37:53] Searching for junctions via segment mapping [2024-04-23 13:40:05] Retrieving sequences for splices [2024-04-23 13:41:31] Indexing splices [2024-04-23 13:41:44] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie (1/2) [2024-04-23 13:42:16] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie (2/2) [2024-04-23 13:42:49] Joining segment hits [2024-04-23 13:44:23] Reporting output tracks ----------------------------------------------- [2024-04-23 13:57:06] A summary of the alignment counts can be found in /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_114W3_08594/align_summary.txt [2024-04-23 13:57:06] Run complete: 01:24:48 elapsed