FastQCFastQC Report
Thu 20 Oct 2022
D2-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD2-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14218881
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAG361100.25395809979702344No Hit
CTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATG348950.24541312357843065No Hit
CTCGATGTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAG320670.22552407605071034No Hit
GTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTT296390.20844818941799992No Hit
GCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATGATC247600.17413465940111603No Hit
CTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCT230570.16215762689061117No Hit
CTCTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATC209720.1474940257253718No Hit
GATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGAC205050.14420966038044766No Hit
CTAGGGATAACAGCGTAATCTTTTTGGAGAGTTCAAATCGATAAAAAGGT204650.14392834429094667No Hit
GGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAGTTTCTTTAG200000.1406580447504976No Hit
GTAGTTTCTTTAGTACGAAAGGATTAGGAAATTGAGACAAATGATCTCTT192710.13553105901934195No Hit
GGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATG179940.12655004286202268No Hit
CTGTTCGACTTTTAATCTCTTACATGATCTGAGTTCAAACCGGCGTAAGC178040.12521379143689296No Hit
ATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTA167190.11758309250917848No Hit
GGTTTCTATCCTCAAAATTATGGTAGTTTCTTTAGTACGAAAGGATTAGG155620.10944602462036217No Hit
GGGATAACAGCGTAATCTTTTTGGAGAGTTCAAATCGATAAAAAGGTTTG153170.10772296357216858No Hit
AGGTTGGTTTCTATCCTCAAAATTATGGTAGTTTCTTTAGTACGAAAGGA150050.10552869807406082No Hit
CTGGAAAGTCTGTTCGACTTTTAATCTCTTACATGATCTGAGTTCAAACC143480.10090808130400698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG182450.060.6401751
ACATGGC191900.057.6053
CATGGCT205700.053.923194
GGCTACG218300.050.5955127
GCTACGA220350.049.6342168
ATGGCTA224000.049.5808075
TGGCTAC261850.042.6114166
CTACGAT255700.042.349639
GACATGG281500.039.303022
GGCCTCC34700.033.1844378
CGCTTGG38200.031.9895293
GCCTCCG39150.029.5325669
TGGCCTC39500.029.5088627
CCGCTTG43900.028.0501142
ACCGCTT45550.026.724481
TACGATG244250.022.22517810-11
ACGATGG242200.021.98637610-11
GCTTGGC58600.021.013654
CGATGGG246850.020.80109212-13
GATGGGA140450.017.50160212-13
Produced by FastQC (version 0.11.5)