FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_72_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_72_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28492134
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGATCGTCCTTCAAAATTATCTGAGCTTTTTCACTCAGAAATGAAAT798120.28011941822258735No Hit
GCTCGAATGGCTTTTAACCCCATGTCTTTATTTTTAAAAATTATGCCACA691730.2427792877851831No Hit
GCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAAATTCAAATT628020.22041873030640668No Hit
GCCACATCTAGTAAACTAAAAACATTACTCGCCTAATTTCGGGATTTATT559560.19639104603396854No Hit
CCTCACCAGGCCCGGACACTGGAAGGATTGACAGATTGAGAGCTCTTTCT492360.17280558907942803No Hit
CCCCCGTCCGTCTCTATTGACCATTACCTCGGGTCCAAAAGAAAAGACCA420400.14754949559060757No Hit
GCCGGTTTGAACTCAGATCATGTAAGAGATTAAAAGTCGAACAGACTTTC398350.13981051752739898No Hit
CTCCGTACTCCCTTCGGTTTCAATTGATAAAACCCGTCACCTATTTAGCA380810.13365443248301445No Hit
CCCCAATTAAAAGACTAATGATTATGCTACCTTAGCACAGTCAGAATACT373470.13107828286922982No Hit
CTCCCTGTCGCCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAA362660.12728425326091758No Hit
GCCATTATTAAACCTGAAATCATTCCGCTACATTTTTCTATTATACCACA327210.1148422227692738No Hit
CCTCCGTACTCCCTTCGGTTTCAATTGATAAAACCCGTCACCTATTTAGC318860.1119115893530474No Hit
CTCGAATGGCTTTTAACCCCATGTCTTTATTTTTAAAAATTATGCCACAT317680.11149743996009566No Hit
CCCCAACAAAATTTACTTTCCATTCAATCAATTAAAACAAATTCAAATTT306890.10771042983301989No Hit
CGCTGTTATCCCTAGGGTAACTTAGTCTTGTAATCTCACTCTAAGGATCA305050.1070646375592646No Hit
GTTATATTTTACTCCCTGTCGCCCCAACAAAATTTACTTTCCATTCAATC299460.10510269255367113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCACC132000.035.9977261
TCGAATG215000.031.2386053
CTCGAAT216950.030.9144972
CTCACCA178200.027.3771042
GCTCGAA246300.027.3641911
CATACCA277350.026.52064194
GGCCCGG197250.024.4233239
CAGGCCC203350.023.6213447
ATGGCTT291100.023.3950547
TCACCAG208500.023.0829723
CGCAGGC67200.022.8705353
CCAGGCC214700.022.4820676
ACCCCAT225850.021.97564716-17
CTGCCAG132400.021.831571
CCCGGAC155350.021.73768810-11
CACCAGG221750.021.449384
ACCAGGC222650.021.3626775
GCCAGGC128200.021.0803431
TAAACCT155950.020.9759549
GCCATTA169500.019.9368721
Produced by FastQC (version 0.11.5)