FastQCFastQC Report
Fri 14 Oct 2022
V350062753_L02_66_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameV350062753_L02_66_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53849550
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCAC2463180.4574188642244921No Hit
CGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1562210.29010641685956523No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCA1486840.27611001391840784No Hit
CCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAAT1341570.2491330011114299No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG1025980.19052712603912197No Hit
CCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAA980350.18205351762456695No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTC943250.17516395215930308No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACT788670.14645804839594762No Hit
CTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAAC703600.1306603304948695No Hit
GCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA664260.12335479126566516No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACAC628400.11669549699115406No Hit
GTTCTTATATGTGATTAAATACTTGTATTTTTTCATATGTTCCTCCTATT604020.1121680682568378No Hit
CTTACCAGGTCCGAACATAAGTGTGTAAGACAGATTGATAGCTCTTTCTC591440.10983192988613646No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGA582830.10823303073099033No Hit
ATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAAT581260.10794147769108563No Hit
CGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC578720.10746979315518884No Hit
GCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC561660.1043017072566066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC285050.043.8753246
GTCCTTT302900.042.3484951
CGCGCGT443250.042.0097271
TTTCGTA318450.039.425365
CCTTTCG318650.039.3899153
TCCTTTC345300.037.0811772
GCGCGTG627300.032.1725462
CGCGTGC626800.032.0684553
CGTACTA392050.031.6167728
TCGTACT418500.030.369677
GCGTGCA680100.029.63544
GCGGTGT1023500.029.278091
CGGTGTG1056350.027.6903062
CGTGCAG728000.027.598735
GTACTAA455150.027.1960959
GCAGCCC742600.026.9130868
GGTGTGT1128700.026.7609523
AATCCCA1094350.026.13375394
CAGCCCA796750.025.1369619
TGCAGCC828650.024.4507127
Produced by FastQC (version 0.11.5)