##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	7301_GTGAAA_R1.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	78021280	
Filtered Sequences	0	
Sequence length	50	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.654112531863102	31.0	31.0	34.0	30.0	34.0
2	32.0047628031737	33.0	31.0	34.0	30.0	34.0
3	32.14580651586336	34.0	31.0	34.0	30.0	34.0
4	35.85902157719022	37.0	35.0	37.0	35.0	37.0
5	35.65733692141426	37.0	35.0	37.0	33.0	37.0
6	35.62873077703929	37.0	35.0	37.0	33.0	37.0
7	35.63207142974327	37.0	35.0	37.0	33.0	37.0
8	35.63166183636054	37.0	35.0	37.0	33.0	37.0
9	37.26506746620922	39.0	37.0	39.0	33.0	39.0
10	37.30952511930079	39.0	37.0	39.0	34.0	39.0
11	37.34307476114209	39.0	37.0	39.0	34.0	39.0
12	37.36496366119602	39.0	37.0	39.0	34.0	39.0
13	37.33951256631524	39.0	37.0	39.0	34.0	39.0
14	38.64269669761891	40.0	38.0	41.0	34.0	41.0
15	38.6452888622181	40.0	38.0	41.0	34.0	41.0
16	38.62802191914821	40.0	38.0	41.0	34.0	41.0
17	38.57603068034772	40.0	38.0	41.0	34.0	41.0
18	38.573535156049736	40.0	38.0	41.0	34.0	41.0
19	38.54815825887501	40.0	38.0	41.0	34.0	41.0
20	38.501754354709384	40.0	38.0	41.0	34.0	41.0
21	38.48174630049648	40.0	38.0	41.0	34.0	41.0
22	38.47403117457186	40.0	38.0	41.0	34.0	41.0
23	38.435365851470266	40.0	38.0	41.0	34.0	41.0
24	38.42545336349263	40.0	38.0	41.0	34.0	41.0
25	38.38492567668718	40.0	38.0	41.0	34.0	41.0
26	38.27890006418762	40.0	38.0	41.0	34.0	41.0
27	38.19026571981388	40.0	38.0	41.0	33.0	41.0
28	38.18246979541992	40.0	38.0	41.0	33.0	41.0
29	38.13662193186269	40.0	38.0	41.0	33.0	41.0
30	38.124279799049695	40.0	38.0	41.0	33.0	41.0
31	38.07691572094178	40.0	38.0	41.0	33.0	41.0
32	38.05588625308378	40.0	38.0	41.0	33.0	41.0
33	37.9881198180804	40.0	37.0	41.0	33.0	41.0
34	37.94453810550147	40.0	37.0	41.0	33.0	41.0
35	37.88442157062791	40.0	37.0	41.0	33.0	41.0
36	37.837814170698046	40.0	37.0	41.0	33.0	41.0
37	37.80326674978929	40.0	37.0	41.0	33.0	41.0
38	37.757714074929304	40.0	37.0	41.0	33.0	41.0
39	37.70761655025398	40.0	37.0	41.0	33.0	41.0
40	37.62036139371207	40.0	37.0	41.0	32.0	41.0
41	37.54292070060886	40.0	37.0	41.0	32.0	41.0
42	37.46108125885656	40.0	37.0	41.0	32.0	41.0
43	37.3912537451321	40.0	37.0	41.0	31.0	41.0
44	37.32997980807288	40.0	37.0	41.0	31.0	41.0
45	37.576385737327044	40.0	37.0	41.0	33.0	41.0
46	37.58055157516001	40.0	37.0	41.0	33.0	41.0
47	37.52849026573263	40.0	37.0	41.0	32.0	41.0
48	37.46388155897981	40.0	37.0	41.0	32.0	41.0
49	37.404665034975075	40.0	37.0	41.0	32.0	41.0
50	37.34639157419617	40.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	112.0
3	96.0
4	100.0
5	245.0
6	349.0
7	474.0
8	622.0
9	898.0
10	1320.0
11	1997.0
12	3110.0
13	5036.0
14	8356.0
15	11986.0
16	16149.0
17	20613.0
18	25212.0
19	31622.0
20	39276.0
21	49545.0
22	63569.0
23	85721.0
24	120492.0
25	175553.0
26	259707.0
27	383201.0
28	554107.0
29	787674.0
30	1086786.0
31	1462255.0
32	1928870.0
33	2541932.0
34	3387670.0
35	4579446.0
36	6304536.0
37	8865200.0
38	1.4458114E7
39	3.0759329E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.646768165045316	31.236798713432545	24.171897137630232	20.944535983891907
2	19.163200795358843	32.91353483420323	27.520122407700594	20.40314196273733
3	19.660034373286425	31.1938753649944	28.29823902283796	20.847851238881216
4	19.7231473259603	32.01237918680647	29.191597471869212	19.072876015364013
5	20.24703323302989	30.44567297125209	29.89824492125681	19.409048874461206
6	19.613076583208642	31.28946684935895	29.830712882359016	19.266743685073394
7	19.902001095085854	29.855156183031088	30.335484114077595	19.907358607805463
8	19.876398883687166	29.687843576753686	30.61963334552661	19.816124194032543
9	19.101963455933554	28.35891249483458	31.811489527783433	20.72763452144843
10	19.78842695223662	29.433469945635345	30.245397153186925	20.53270594894111
11	20.07609975124735	30.198407408850507	29.158260413056542	20.5672324268456
12	19.685241000916673	30.16710441048904	29.783625698014692	20.364028890579597
13	20.037574906867	30.111075682002298	29.789654142543636	20.061695268587066
14	19.74729300502509	29.98408546076934	30.102391905247078	20.16622962895849
15	19.665168120882235	29.889390135888338	30.095293638617775	20.350148104611648
16	19.795236926016884	29.668304306939103	30.03872508633925	20.497733680704762
17	19.853982144358564	29.77710183683221	29.916430491783778	20.452485527025445
18	19.729885231311254	29.976253914316707	29.787656649570476	20.506204204801563
19	19.912839163879394	29.972642079186603	29.549278094386555	20.56524066254745
20	19.99580499063845	30.117246217954897	29.687589078261727	20.199359713144926
21	20.17209535654888	29.80824334079113	29.774332079658265	20.245329223001722
22	20.145257806588152	30.05762145917114	29.7097894318063	20.087331302434414
23	19.93488212363194	29.798780919753952	29.987448680506763	20.27888827610734
24	19.961573727147496	29.85334172046643	29.992525867810784	20.192558684575292
25	19.970303542736286	29.77630218916395	30.00775557316785	20.245638694931912
26	19.922706110191648	30.082637769046244	29.658409265411017	20.336246855351092
27	19.936988551827145	29.900139528316398	29.833155988268228	20.32971593158823
28	20.224181068168587	29.697731904779413	29.847064338510815	20.231022688541188
29	20.36889446512174	29.620110021785933	29.892871297890984	20.11812421520134
30	19.943838078717768	29.779736420228847	29.988561080226127	20.287864420827255
31	20.062591898005262	29.841634231071318	29.653709859669053	20.442064011254367
32	19.997472484429892	29.995851131896323	29.74936453234297	20.25731185133082
33	20.050665664546905	29.933751407308367	29.802778165136484	20.212804763008247
34	20.132472833052724	29.968359914115737	29.599507980386893	20.299659272444647
35	20.000519089151062	29.87199133364641	29.862249119727334	20.26524045747519
36	20.02585448482773	29.886809085931425	29.908663380041954	20.178673049198885
37	20.050752820256218	29.727160333693575	29.98134739650516	20.240739449545046
38	20.112578260700158	29.667626575723954	29.91579989459286	20.303995268983027
39	20.153203074853423	29.73072730926742	29.786450568357758	20.329619047521394
40	20.15243149048567	29.732089758076256	29.682896768676443	20.432581982761626
41	20.34682722368921	29.81983194288025	29.557986481394146	20.2753543520364
42	20.25101892164964	29.955348039334435	29.70887403566287	20.08475900335306
43	20.195160802575977	29.766084894322347	29.75398390787982	20.28477039522186
44	20.117023610383082	29.82804138112163	29.73104167971141	20.323893328783882
45	19.967836363831672	29.94506901028771	29.92938418392666	20.157710441953952
46	19.956092067910085	29.772136783304298	29.81649002962711	20.4552811191585
47	19.996235251186924	29.84214438738591	29.85367477995719	20.30794558146998
48	19.870456520212308	29.7764644368936	30.10489366211328	20.248185380780814
49	20.16025015909584	29.753269260018005	29.792832832689122	20.293647748197035
50	20.157526786059666	29.970563654076138	29.780841481492264	20.09106807837193
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.59130414893722
2	39.56634275809618
3	40.50788561216764
4	38.796023341324315
5	39.656082107491095
6	38.87982026828204
7	39.809359702891314
8	39.692523077719706
9	39.82959797738199
10	40.321132901177734
11	40.64333217809295
12	40.04926989149627
13	40.099270175454066
14	39.913522633983575
15	40.01531622549388
16	40.292970606721646
17	40.30646767138401
18	40.23608943611281
19	40.47807982642684
20	40.19516470378338
21	40.417424579550605
22	40.23258910902256
23	40.21377039973928
24	40.154132411722784
25	40.2159422376682
26	40.25895296554275
27	40.266704483415374
28	40.45520375670977
29	40.48701868032308
30	40.23170249954502
31	40.50465590925963
32	40.254784335760704
33	40.26347042755515
34	40.432132105497374
35	40.265759546626256
36	40.20452753402661
37	40.29149226980127
38	40.416573529683184
39	40.48282212237482
40	40.5850134732473
41	40.622181575725605
42	40.3357779250027
43	40.47993119779784
44	40.44091693916696
45	40.125546805785625
46	40.41137318706859
47	40.304180832656904
48	40.11864190099312
49	40.45389790729288
50	40.24859486443159
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1715.0
1	2866.5
2	4018.0
3	6197.0
4	8376.0
5	11738.0
6	15100.0
7	21340.0
8	27580.0
9	38929.0
10	50278.0
11	71924.0
12	93570.0
13	129715.0
14	165860.0
15	224434.0
16	283008.0
17	372427.5
18	461847.0
19	590537.0
20	719227.0
21	888705.0
22	1058183.0
23	1276078.5
24	1493974.0
25	1766975.0
26	2039976.0
27	2390875.0
28	2741774.0
29	3228921.0
30	3716068.0
31	4288318.5
32	4860569.0
33	5437505.5
34	6014442.0
35	6409241.5
36	6804041.0
37	6716846.0
38	6629651.0
39	6383725.0
40	6137799.0
41	5808362.0
42	5478925.0
43	5265712.0
44	5052499.0
45	4831082.0
46	4609665.0
47	4415244.0
48	4220823.0
49	4000390.5
50	3779958.0
51	3480017.5
52	3180077.0
53	2849500.0
54	2518923.0
55	2201443.5
56	1883964.0
57	1616392.5
58	1348821.0
59	1137728.5
60	926636.0
61	767353.5
62	608071.0
63	497614.5
64	387158.0
65	314332.5
66	241507.0
67	194972.0
68	148437.0
69	120826.5
70	93216.0
71	77840.0
72	62464.0
73	53665.5
74	44867.0
75	38752.0
76	32637.0
77	28244.0
78	23851.0
79	20827.0
80	17803.0
81	15153.0
82	12503.0
83	10604.0
84	8705.0
85	7160.5
86	5616.0
87	4550.5
88	3485.0
89	2701.5
90	1918.0
91	1448.0
92	978.0
93	706.0
94	434.0
95	308.5
96	183.0
97	131.5
98	80.0
99	50.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02912923243504849
2	0.0034080445745058273
3	0.015206107872108736
4	0.0
5	0.00430139059497614
6	1.3329696718638811E-4
7	0.0
8	1.7943822505859941E-4
9	2.9479136973912756E-5
10	0.0
11	0.0
12	0.0
13	7.946549966880831E-5
14	2.1917097489300354E-4
15	9.638396088861911E-4
16	3.575947485096374E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.2817016075614242E-6
24	2.3326969257617922E-4
25	0.003263212292851386
26	0.02069819926050944
27	0.021842758796061793
28	0.01723119641205579
29	0.009792200281769282
30	0.001026642987656701
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	8.843741092173827E-5
42	8.971911252929971E-5
43	0.003649004476727375
44	0.005825333806366673
45	0.01227613799722332
46	0.01147379279088987
47	0.0240088345128406
48	0.013536050677456201
49	0.012977228776559421
50	0.0018020724602313626
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7.802128E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	49.347342220520304
#Duplication Level	Relative count
1	100.0
2	49.33519811757591
3	17.025921130966445
4	4.961208077791743
5	1.7368953232345186
6	0.8882924939284264
7	0.6624394023870498
8	0.507460711956984
9	0.41863146256414135
10++	13.462356243089745
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTC	18260625	3.235353	4.150886	7
CACAC	8308505	3.0926752	3.5411074	12
>>END_MODULE