##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename 7300_CAGATC_R1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58867511 Filtered Sequences 0 Sequence length 50 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60631226620062 31.0 31.0 34.0 30.0 34.0 2 31.95687731726928 33.0 31.0 34.0 30.0 34.0 3 32.103155253158235 34.0 31.0 34.0 30.0 34.0 4 35.81365590605657 37.0 35.0 37.0 35.0 37.0 5 35.60365952961728 37.0 35.0 37.0 33.0 37.0 6 35.571313640218285 37.0 35.0 37.0 33.0 37.0 7 35.57538806082697 37.0 35.0 37.0 33.0 37.0 8 35.57534401276113 37.0 35.0 37.0 33.0 37.0 9 37.18408984541575 39.0 37.0 39.0 33.0 39.0 10 37.23157715976814 39.0 37.0 39.0 33.0 39.0 11 37.26526282043758 39.0 37.0 39.0 33.0 39.0 12 37.2894816123617 39.0 37.0 39.0 34.0 39.0 13 37.26918408356011 39.0 37.0 39.0 33.0 39.0 14 38.563678732739355 40.0 38.0 41.0 34.0 41.0 15 38.56331483082408 40.0 38.0 41.0 34.0 41.0 16 38.54867147347201 40.0 38.0 41.0 34.0 41.0 17 38.488298222766716 40.0 38.0 41.0 34.0 41.0 18 38.4852829772266 40.0 38.0 41.0 34.0 41.0 19 38.46588560538936 40.0 38.0 41.0 34.0 41.0 20 38.422253677414695 40.0 38.0 41.0 34.0 41.0 21 38.39520201559057 40.0 38.0 41.0 34.0 41.0 22 38.38885170463551 40.0 38.0 41.0 34.0 41.0 23 38.35283197212126 40.0 38.0 41.0 34.0 41.0 24 38.34409548927591 40.0 38.0 41.0 34.0 41.0 25 38.2996812792034 40.0 38.0 41.0 33.0 41.0 26 38.19142377193423 40.0 38.0 41.0 33.0 41.0 27 38.097931166140185 40.0 38.0 41.0 33.0 41.0 28 38.08032970852123 40.0 38.0 41.0 33.0 41.0 29 38.03167750713972 40.0 37.0 41.0 33.0 41.0 30 38.0108791418071 40.0 37.0 41.0 33.0 41.0 31 37.975560865822914 40.0 37.0 41.0 33.0 41.0 32 37.952410863778496 40.0 37.0 41.0 33.0 41.0 33 37.88953900225202 40.0 37.0 41.0 33.0 41.0 34 37.84149781702168 40.0 37.0 41.0 33.0 41.0 35 37.783499339729175 40.0 37.0 41.0 33.0 41.0 36 37.725745581463435 40.0 37.0 41.0 32.0 41.0 37 37.698823057084915 40.0 37.0 41.0 32.0 41.0 38 37.64798836152593 40.0 37.0 41.0 32.0 41.0 39 37.590566144371216 40.0 37.0 41.0 32.0 41.0 40 37.50904284028587 40.0 37.0 41.0 32.0 41.0 41 37.429988011553604 40.0 37.0 41.0 31.0 41.0 42 37.342299116400554 40.0 37.0 41.0 31.0 41.0 43 37.2696583009939 39.0 37.0 41.0 31.0 41.0 44 37.21316615543674 39.0 36.0 41.0 31.0 41.0 45 37.43260913477385 40.0 37.0 41.0 32.0 41.0 46 37.4222722785069 40.0 37.0 41.0 32.0 41.0 47 37.370595386647146 40.0 37.0 41.0 32.0 41.0 48 37.30219434621586 40.0 37.0 41.0 32.0 41.0 49 37.22463229335448 40.0 37.0 41.0 31.0 41.0 50 37.16398617566827 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83.0 3 66.0 4 79.0 5 163.0 6 209.0 7 323.0 8 462.0 9 664.0 10 1020.0 11 1505.0 12 2385.0 13 3989.0 14 6407.0 15 9552.0 16 12718.0 17 16069.0 18 19824.0 19 25321.0 20 32029.0 21 40220.0 22 52271.0 23 71775.0 24 102600.0 25 149222.0 26 222543.0 27 327586.0 28 473493.0 29 665914.0 30 910155.0 31 1206451.0 32 1568088.0 33 2020161.0 34 2631166.0 35 3473697.0 36 4677625.0 37 6516637.0 38 1.0687125E7 39 2.2937914E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.820489037533626 31.602644421204403 23.607716116900484 20.969150424361484 2 18.578100741623054 33.62997507934131 27.506247747093255 20.285676431942388 3 18.92911671700582 31.29953914420009 28.941059204817066 20.83028493397703 4 19.072399714674535 32.3490303505443 29.553488340962808 19.025081593818364 5 20.092979715976686 30.68107404970385 30.18371480811456 19.042231426204903 6 19.640230357539206 31.582377465467076 29.973911217415477 18.803480959578238 7 19.450034162307286 30.38605284330775 30.535191134546185 19.628721859838784 8 19.51907702242408 30.282749588231272 30.785151690692448 19.4130216986522 9 19.21010123532864 28.40395462398917 31.956215631605033 20.429728509077158 10 19.439216650420295 30.024490079086235 30.29338712825823 20.24290614223523 11 20.13190603557198 30.47192703629002 29.156595392660652 20.239571535477356 12 19.250192181558347 30.450815221319615 29.907454385153127 20.391538211968907 13 19.713060900150055 30.727228078446174 29.96674530318729 19.592965718216483 14 19.342601139269572 30.125125181834616 30.365482458218317 20.166791220677492 15 19.211628350349415 30.472957859873752 30.33727732860389 19.978136461172944 16 19.406590755818595 29.7039358013702 30.26424687390113 20.625226568910072 17 19.848245324997688 29.90056943294239 30.095121568839563 20.156063673220363 18 19.289650279251656 30.097504886863657 30.37858862420733 20.234256209677355 19 19.560966319775268 30.09608984487216 29.633940188162534 20.709003647190045 20 19.614337015200118 30.245314771334563 30.288934757238167 19.851413456227153 21 20.300746195978967 29.92171182505066 29.9130703861422 19.864471592828174 22 19.88952106366447 30.68670594888919 29.813254717020392 19.61051827042594 23 19.5654034022264 30.41233898949796 30.179431231600738 19.842826376674903 24 19.588310531032814 30.042694783472523 30.190348715882187 20.178645969612475 25 19.587312524239533 29.94636936574683 30.647172432257136 19.819145677756502 26 19.524676002148123 30.33134071633592 29.747921407724466 20.396061873791492 27 19.535640138055548 30.03843541801427 29.993381992256506 20.432542451673676 28 19.947471221052425 30.215703211464483 30.000382280792547 19.836443286690546 29 20.502754584939563 29.685888515484294 30.103878616718404 19.70747828285774 30 19.53342066587624 29.910193233231315 30.62318589829997 19.933200202592474 31 19.70151413400169 30.00584142244438 29.785381957120627 20.507262486433305 32 19.601246602731344 30.605075183151538 29.91635063354386 19.877327580573265 33 19.66629436736335 30.110938442768543 29.997612774897174 20.225154414970934 34 19.75005024418308 30.125019214758375 29.7125726956589 20.412357845399647 35 19.607086836064802 30.50039095418863 29.98447989418136 19.908042315565204 36 20.04584837976248 30.08902142983419 30.084254793786 19.78087539661733 37 19.654083896973322 30.27493722301254 30.217312907963784 19.853665972050354 38 19.772417420536094 29.691583189239985 30.586621880446074 19.949377509777847 39 19.80530483104679 29.788894930516086 29.94034689185347 20.465453346583654 40 19.82047279016094 30.329215040194242 29.776913788660096 20.073398380984717 41 20.01751629907308 29.946081585517074 30.132281234226216 19.904120881183623 42 19.980299474755718 30.09860149237611 29.809504625186346 20.111594407681828 43 19.875586164335342 29.92607973340091 30.289893278075898 19.90844082418785 44 19.75861643086036 30.03005912443244 29.809776741264056 20.40154770344314 45 20.012561293133615 30.163685188741006 30.065392934055147 19.75836058407023 46 19.527628590735667 29.91331478954327 30.443086152172054 20.115970467549005 47 19.538699071292427 30.443829455379895 30.068916190434148 19.948555282893526 48 19.411697796655165 29.89970416777276 30.800585293336873 19.8880127422352 49 20.2851332334076 29.836839840466773 29.95848841394263 19.919538512183003 50 19.802347197527908 30.174145357077382 29.87029489256156 20.153212552833143 >>END_MODULE >>Per base GC content warn #Base %GC 1 44.78963946189511 2 38.86377717356544 3 39.75940165098285 4 38.097481308492895 5 39.13521114218159 6 38.44371131711745 7 39.078756022146074 8 38.93209872107628 9 39.639829744405795 10 39.68212279265553 11 40.37147757104933 12 39.641730393527254 13 39.30602661836654 14 39.509392359947064 15 39.18976481152236 16 40.03181732472867 17 40.00430899821804 18 39.523906488929015 19 40.269969966965306 20 39.46575047142727 21 40.16521778880714 22 39.50003933409042 23 39.4082297789013 24 39.76695650064529 25 39.40645820199604 26 39.920737875939615 27 39.968182589729224 28 39.78391450774297 29 40.2102328677973 30 39.466620868468716 31 40.208776620434996 32 39.47857418330461 33 39.89144878233428 34 40.162408089582726 35 39.515129151630006 36 39.82672377637981 37 39.50774986902368 38 39.72179493031393 39 40.27075817763045 40 39.89387117114566 41 39.9216371802567 42 40.09189388243754 43 39.784026988523195 44 40.1601641343035 45 39.77092187720385 46 39.643599058284664 47 39.48725435418596 48 39.299710538890366 49 40.204671745590595 50 39.955559750361054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1505.0 1 2468.0 2 3431.0 3 5246.5 4 7062.0 5 9817.0 6 12572.0 7 17615.0 8 22658.0 9 32147.5 10 41637.0 11 58806.5 12 75976.0 13 104718.0 14 133460.0 15 179881.0 16 226302.0 17 296427.5 18 366553.0 19 464591.5 20 562630.0 21 690338.0 22 818046.0 23 983010.5 24 1147975.0 25 1353775.5 26 1559576.0 27 1838721.5 28 2117867.0 29 2523635.5 30 2929404.0 31 3415168.5 32 3900933.0 33 4393743.0 34 4886553.0 35 5216613.0 36 5546673.0 37 5419627.5 38 5292582.0 39 5010211.5 40 4727841.0 41 4388488.0 42 4049135.0 43 3841361.0 44 3633587.0 45 3435112.0 46 3236637.0 47 3076854.0 48 2917071.0 49 2749679.0 50 2582287.0 51 2515638.0 52 2448989.0 53 2080150.0 54 1711311.0 55 1489670.0 56 1268029.0 57 1086235.5 58 904442.0 59 761734.5 60 619027.0 61 512774.5 62 406522.0 63 332252.0 64 257982.0 65 210488.5 66 162995.0 67 131734.5 68 100474.0 69 81494.5 70 62515.0 71 51927.5 72 41340.0 73 34765.0 74 28190.0 75 23609.5 76 19029.0 77 16028.0 78 13027.0 79 10946.0 80 8865.0 81 7338.0 82 5811.0 83 4790.5 84 3770.0 85 3030.5 86 2291.0 87 1884.0 88 1477.0 89 1107.0 90 737.0 91 558.0 92 379.0 93 304.0 94 229.0 95 153.0 96 77.0 97 57.0 98 37.0 99 25.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028839337202485087 2 0.003213996936272709 3 0.014868982654965657 4 0.0 5 0.004100733934546681 6 1.1041744231380872E-4 7 0.0 8 2.072450455736102E-4 9 3.057713787151626E-5 10 0.0 11 0.0 12 0.0 13 6.285300562478342E-5 14 2.446171029721301E-4 15 9.410963544900004E-4 16 3.5843200504944063E-4 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 2.2423234439111924E-4 25 0.003229285505208467 26 0.021088032752055712 27 0.02198326339973844 28 0.01741368001782851 29 0.009903595210607765 30 9.614811130710112E-4 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 9.003268373279787E-5 42 7.644284467879064E-5 43 0.003725314630679731 44 0.00598122791364493 45 0.012449991303352372 46 0.011612517471649176 47 0.024516069653429038 48 0.01382086631792535 49 0.013248394347775295 50 0.0017921600252472029 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 5.8867511E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 56.46673814216758 #Duplication Level Relative count 1 100.0 2 60.00924890303708 3 23.806246235911555 4 7.702400412974275 5 2.7295018497806076 6 1.2539791792136281 7 0.9410221113309817 8 0.7108749892454616 9 0.5936505205196593 10++ 17.343844102211133 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 260355 0.4422728183632564 TruSeq Adapter, Index 7 (100% over 50bp) >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTC 16088875 3.700611 4.7925706 7 GAAAA 15280975 3.3866634 5.028591 1 CACAC 6619460 3.3098693 9.483007 12 GTGTG 5685630 3.0525823 4.18752 22 CCAGG 3880850 3.026293 4.4547396 35 CCTGG 3808760 3.0090587 3.4274585 30 CTGAA 7037735 2.3914587 6.7106266 19 TCCAG 4436990 2.2817314 8.840894 25 GGAAG 3697980 1.9343162 8.471005 5 GCACA 3512620 1.7829713 8.233632 11 CTCCA 3514940 1.7806112 8.185356 24 CCAGA 3506930 1.7800831 8.133369 33 CACGT 3167230 1.6287547 8.166207 14 CACCA 3159570 1.5798515 7.865584 31 GAAGA 4602490 1.567057 5.794449 6 AGAGC 2808540 1.4471669 7.99865 8 AAGAG 4226720 1.4391146 5.7200503 7 CCAGT 2728070 1.4029158 7.8294454 26 GTCTG 2645255 1.3990442 8.109376 17 ACCAG 2746880 1.3942891 7.7342534 32 GAGCA 2690635 1.3864136 7.9658093 9 TCTGA 3997840 1.3763164 5.786311 18 AGCAC 2585105 1.3121737 7.7893753 10 CATCT 3818015 1.294809 5.489028 39 CAGTC 2385675 1.2268384 7.692667 27 TCACC 2262210 1.1459986 7.4473147 30 CAGAT 3355450 1.1401994 5.3718 34 ACTCC 2215945 1.1225617 7.550242 23 ACGTC 2158865 1.1102009 7.625109 15 AACTC 3287105 1.1003193 5.33848 22 AGTCA 3022850 1.0271802 5.2718315 28 GTCAC 1983325 1.019929 7.4112263 29 GAACT 2963330 1.0069549 5.3392434 21 ATCTC 2952265 1.0012058 5.2280364 40 TCATC 2803425 0.9507295 5.180287 38 TGAAC 2620290 0.8903882 5.233569 20 AGATC 2474970 0.8410077 5.063976 35 GTATG 2285385 0.79868805 5.0803127 45 TCTCG 1456915 0.75905496 7.477961 41 CGGAA 1343235 0.69213367 6.972965 4 ACACG 844170 0.42849234 6.9197874 13 CGTCT 703765 0.36666265 7.011708 16 CTCGT 693735 0.361437 6.784606 42 TCGGA 571685 0.2984405 6.940157 3 ATCGG 515180 0.2689428 6.9302797 2 GATCG 511795 0.26717573 6.908372 1 >>END_MODULE