##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	7299_CGATGT_R1.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	43649998	
Filtered Sequences	0	
Sequence length	50	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84794015339932	33.0	31.0	34.0	30.0	34.0
2	32.18444438416698	34.0	31.0	34.0	30.0	34.0
3	32.320137952812736	34.0	31.0	34.0	30.0	34.0
4	36.00388630029261	37.0	35.0	37.0	35.0	37.0
5	35.8282286061044	37.0	35.0	37.0	35.0	37.0
6	35.80325350759466	37.0	35.0	37.0	35.0	37.0
7	35.80861614243373	37.0	35.0	37.0	35.0	37.0
8	35.808055386394294	37.0	35.0	37.0	35.0	37.0
9	37.47213740994902	39.0	37.0	39.0	34.0	39.0
10	37.51446593422524	39.0	37.0	39.0	35.0	39.0
11	37.54445166297602	39.0	37.0	39.0	35.0	39.0
12	37.56453125152491	39.0	37.0	39.0	35.0	39.0
13	37.5439427282448	39.0	37.0	39.0	35.0	39.0
14	38.894284347962625	40.0	38.0	41.0	35.0	41.0
15	38.89312173622551	40.0	38.0	41.0	35.0	41.0
16	38.874992961053515	40.0	38.0	41.0	35.0	41.0
17	38.81543075901172	40.0	38.0	41.0	35.0	41.0
18	38.81049618833889	40.0	38.0	41.0	35.0	41.0
19	38.78127339204002	40.0	38.0	41.0	34.0	41.0
20	38.73409930053147	40.0	38.0	41.0	34.0	41.0
21	38.70981228910939	40.0	38.0	41.0	34.0	41.0
22	38.70078514092945	40.0	38.0	41.0	34.0	41.0
23	38.66503961810032	40.0	38.0	41.0	34.0	41.0
24	38.65199317993096	40.0	38.0	41.0	34.0	41.0
25	38.60902843569431	40.0	38.0	41.0	34.0	41.0
26	38.514724536757136	40.0	38.0	41.0	34.0	41.0
27	38.42764618225183	40.0	38.0	41.0	34.0	41.0
28	38.41257074055307	40.0	38.0	41.0	34.0	41.0
29	38.360767805762556	40.0	38.0	41.0	34.0	41.0
30	38.3395750900149	40.0	38.0	41.0	34.0	41.0
31	38.30095902868083	40.0	38.0	41.0	34.0	41.0
32	38.27659634715218	40.0	38.0	41.0	34.0	41.0
33	38.2119799638937	40.0	38.0	41.0	34.0	41.0
34	38.166773111879635	40.0	38.0	41.0	34.0	41.0
35	38.095286441937525	40.0	38.0	41.0	33.0	41.0
36	38.04357663429904	40.0	38.0	41.0	33.0	41.0
37	38.00511333356762	40.0	38.0	41.0	33.0	41.0
38	37.950416309297424	40.0	38.0	41.0	33.0	41.0
39	37.89714886126684	40.0	37.0	41.0	33.0	41.0
40	37.81167575311229	40.0	37.0	41.0	33.0	41.0
41	37.73505586414918	40.0	37.0	41.0	33.0	41.0
42	37.643719594213955	40.0	37.0	41.0	33.0	41.0
43	37.56919826204803	40.0	37.0	41.0	32.0	41.0
44	37.50750352840795	40.0	37.0	41.0	32.0	41.0
45	37.7468845932135	40.0	37.0	41.0	33.0	41.0
46	37.74667114532285	40.0	37.0	41.0	33.0	41.0
47	37.69852495754983	40.0	37.0	41.0	33.0	41.0
48	37.63610900967281	40.0	37.0	41.0	33.0	41.0
49	37.568137116524035	40.0	37.0	41.0	33.0	41.0
50	37.50720187890959	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75.0
3	56.0
4	69.0
5	150.0
6	195.0
7	306.0
8	414.0
9	547.0
10	929.0
11	1258.0
12	1779.0
13	2936.0
14	4478.0
15	6514.0
16	8792.0
17	11135.0
18	13651.0
19	17374.0
20	21435.0
21	26357.0
22	33196.0
23	42807.0
24	58073.0
25	82571.0
26	118811.0
27	173453.0
28	250084.0
29	354443.0
30	492050.0
31	670721.0
32	907230.0
33	1226327.0
34	1691903.0
35	2375613.0
36	3423036.0
37	5051963.0
38	8563007.0
39	1.801626E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.869989037403954	30.978832551123375	23.229818481243896	20.921359930228775
2	19.689035352664416	32.60783178589408	27.007209836438374	20.69592302500314
3	19.876059009093012	30.46661392572197	28.2292778873817	21.428049177803317
4	19.837391516031687	31.423946456996397	29.158001335990896	19.580660690981016
5	20.6999501101627	29.886571642594483	29.51692726256002	19.896550984682793
6	20.276010999972577	30.834191314913312	29.285331909031598	19.60446577608251
7	20.087068503416656	29.75519494869164	29.87306207894901	20.2846744689427
8	20.205798287034895	29.56102605992963	30.21873393324882	20.01444171978665
9	19.87099055685772	27.8475251639846	31.25993886412281	21.021545415034872
10	20.223487753653504	29.275554605981885	29.71162564543531	20.7893319949293
11	20.674747797239306	29.861213281155248	28.672931439767762	20.791107481837685
12	19.96121053659613	29.734755085212143	29.39095438217431	20.913079996017412
13	20.33675166328866	29.95074910704406	29.444457808332974	20.26804142133431
14	20.011543209015315	29.4739390853685	29.806842429240454	20.70767527637573
15	19.90305758640538	29.815944581425384	29.624066644329705	20.65693118783953
16	20.116465463226106	29.112521577966476	29.654188960557708	21.116823998249707
17	20.443270123402986	29.333792867527738	29.502365612937716	20.720571396131565
18	20.007446506641305	29.482862748355682	29.654617624495653	20.85507312050736
19	20.386887532045247	29.412997911248475	29.036887928379745	21.163226628326534
20	20.388988333974265	29.525417160385665	29.524012807514904	20.561581698125163
21	20.8973388727303	29.272681753616574	29.235007066896085	20.594972306757036
22	20.644729468257935	29.846548904767417	29.197584384769044	20.3111372422056
23	20.240239186265256	29.7536852120818	29.481950949917568	20.524124651735377
24	20.255748087979846	29.401373739499004	29.601028235891274	20.741849936629876
25	20.324785018705654	29.278176728146732	29.9326381062012	20.464400146946414
26	20.184068049350067	29.66476917114292	29.23490997028164	20.916252809225373
27	20.208619163153987	29.376014167397525	29.345718898862653	21.06964777058583
28	20.70762846383694	29.483633340730552	29.368417760334044	20.440320435098467
29	21.044173537833235	29.08299450913835	29.519448965880812	20.353382987147604
30	20.237087619655448	29.23340124002508	29.903329471442362	20.626181668877113
31	20.349180772012865	29.449192643720167	29.163701221704525	21.037925362562447
32	20.29634228161935	29.756111329031448	29.43174247109931	20.51580391824989
33	20.315061641010843	29.472800892224555	29.388186913548083	20.82395055321652
34	20.39377642124978	29.493142244817516	29.08108037026714	21.032000963665563
35	20.612122364816603	29.33243433367397	29.34888794267528	20.70655535883415
36	20.24283483357777	29.81757066747174	29.44257408671588	20.497020412234612
37	20.3378222377009	29.21147900167143	29.940883387898438	20.509815372729225
38	20.86433314384115	29.057320919006685	29.42829000816907	20.65005592898309
39	20.492649278013715	29.132269376048995	29.665094142730545	20.709987203206744
40	20.500986048155145	29.52393491518602	29.139121610039936	20.835957426618897
41	20.686383848925	29.270852069036778	29.48311348542469	20.559650596613526
42	20.74268126309423	29.35441850448855	29.214051791582808	20.688848440834413
43	20.507107966825007	29.280848833532723	29.62737228248163	20.58467091716064
44	20.41778740766187	29.29444328286488	29.212534740278826	21.07523456919442
45	20.599303960731604	29.424428608271562	29.461727555056903	20.51453987593993
46	20.228485080022153	29.212429600817398	29.67302033280393	20.886064986356526
47	20.21669933268921	29.661425223620103	29.44555495375809	20.676320489932593
48	20.144970040685827	29.223055806511706	30.01212074276095	20.619853410041518
49	20.81024863787657	29.242789147811006	29.35443474065696	20.59252747365547
50	20.455165584424957	29.4631811697686	29.28723756629386	20.79441567951258
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.79134896763273
2	40.38495837766755
3	41.30410818689633
4	39.4180522070127
5	40.59650109484549
6	39.88047677605509
7	40.37174297235936
8	40.22024000682155
9	40.89253597189259
10	41.01281974858281
11	41.46585527907699
12	40.874290532613536
13	40.60479308462297
14	40.719218485391046
15	40.55998877424491
16	41.23328946147581
17	41.16384151953455
18	40.862519627148664
19	41.550114160371784
20	40.950570032099435
21	41.49231117948734
22	40.95586671046354
23	40.764363838000634
24	40.99759802460972
25	40.789185165652064
26	41.10032085857544
27	41.278266933739815
28	41.14794889893541
29	41.397556524980835
30	40.86326928853256
31	41.387106134575305
32	40.81214619986924
33	41.13901219422736
34	41.42577738491535
35	41.31867772365075
36	40.739855245812386
37	40.847637610430134
38	41.514389072824244
39	41.20263648122046
40	41.33694347477404
41	41.24603444553853
42	41.431529703928646
43	41.09177888398564
44	41.49302197685629
45	41.113843836671535
46	41.11455006637868
47	40.8930198226218
48	40.76482345072735
49	41.40277611153204
50	41.249581263937536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	876.0
1	1489.0
2	2102.0
3	3233.0
4	4364.0
5	6044.5
6	7725.0
7	10780.5
8	13836.0
9	19953.5
10	26071.0
11	36717.0
12	47363.0
13	65659.5
14	83956.0
15	113943.0
16	143930.0
17	188048.0
18	232166.0
19	295899.5
20	359633.0
21	444605.5
22	529578.0
23	640653.0
24	751728.0
25	890113.0
26	1028498.0
27	1214841.0
28	1401184.0
29	1671423.0
30	1941662.0
31	2263224.0
32	2584786.0
33	2923428.0
34	3262070.0
35	3501641.0
36	3741212.0
37	3692388.5
38	3643565.0
39	3501961.5
40	3360358.0
41	3176291.0
42	2992224.0
43	2902328.0
44	2812432.0
45	2684473.5
46	2556515.0
47	2468205.5
48	2379896.0
49	2268787.0
50	2157678.0
51	2099204.5
52	2040731.0
53	1779068.5
54	1517406.0
55	1337759.0
56	1158112.0
57	1005785.5
58	853459.0
59	731729.0
60	609999.0
61	514920.5
62	419842.0
63	352765.0
64	285688.0
65	240504.0
66	195320.0
67	163807.0
68	132294.0
69	113275.5
70	94257.0
71	82188.0
72	70119.0
73	62569.5
74	55020.0
75	48955.5
76	42891.0
77	38121.0
78	33351.0
79	29546.5
80	25742.0
81	22115.5
82	18489.0
83	15763.0
84	13037.0
85	10788.0
86	8539.0
87	6800.0
88	5061.0
89	3913.5
90	2766.0
91	2083.0
92	1400.0
93	1032.5
94	665.0
95	463.5
96	262.0
97	185.0
98	108.0
99	70.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028691868439489964
2	0.0032875144690728275
3	0.014861398160888805
4	0.0
5	0.004192440054636429
6	1.626575103164953E-4
7	0.0
8	1.649484611660234E-4
9	3.436426274292155E-5
10	0.0
11	0.0
12	0.0
13	6.414662378678688E-5
14	2.084765273070574E-4
15	8.545246668739825E-4
16	3.3906072573015926E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	2.107674781565855E-4
25	0.0032829325673737722
26	0.020536083415169916
27	0.021809852087507544
28	0.017097366190028232
29	0.00963803022396473
30	9.095074872626569E-4
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	1.1225659162687704E-4
42	1.0996564077734896E-4
43	0.0036219932931039313
44	0.005809851354403269
45	0.012219931831382902
46	0.011363116213659392
47	0.023738832702810204
48	0.013305842534059222
49	0.012925544693037557
50	0.0017961054660300326
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4.3649998E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	63.053086875455485
#Duplication Level	Relative count
1	100.0
2	75.93180828444808
3	38.56981837743679
4	14.86410328662551
5	5.371826728488357
6	2.1428388451981455
7	1.1834925839588084
8	0.8684068960217229
9	0.7480082998181212
10++	16.48692266311448
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	148359	0.3398831770851399	TruSeq Adapter, Index 2 (100% over 50bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA	56251	0.12886827623680533	No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT	44206	0.10127377325423932	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTC	10572415	3.4592478	4.4014864	7
GAAAA	10042165	3.1816974	4.680209	1
CACAC	4812595	3.1057532	7.6413608	12
CCAGG	3158215	2.9839525	5.2468743	35
TCCAG	3398660	2.2393997	7.0460486	25
CTGAA	4844460	2.2252269	5.5512257	19
GGAAG	2932380	1.951234	6.7177105	5
CTCCA	2781795	1.8143255	6.510124	24
GCACA	2580340	1.6822804	6.4364166	11
AGAGC	2241630	1.4764516	6.267917	8
GAGCA	2119040	1.3957076	6.186269	9
GTCTG	2070855	1.3931885	6.2630796	17
CCAGT	2101340	1.3845868	6.1331205	26
CACGT	2048690	1.3498956	6.112833	14
AGCAC	1992050	1.2987384	6.0724688	10
CAGTC	1851555	1.2200019	5.9405274	27
TCACC	1740230	1.1350023	5.785668	30
ACTCC	1720500	1.1221341	5.828589	23
GTCAC	1515260	0.99841493	5.6815963	29
ACGTC	1419145	0.9350841	5.6572995	15
TCTCG	987500	0.6576025	5.546242	41
CGGAA	921620	0.6070258	5.1887307	4
CACCG	509030	0.47605792	7.161794	31
ACACG	632410	0.41230655	5.192997	13
CGTCT	523320	0.34849274	5.211828	16
CGATG	509065	0.3388683	5.029291	34
CTCGT	494075	0.32901767	5.011469	42
TCGGA	431180	0.28702274	5.1526284	3
ATCGG	371915	0.24757193	5.1405454	2
GATCG	358380	0.23856214	5.1285477	1
>>END_MODULE