Flowcell  AD26VJACXX
Run Start  2013-07-01
Read Length  50 single end
HiSeq Sequencing Kit Trueseq v3

Sequencer Software	HiSeq Control Software	1.5.15.1
Gerald version     1.8

Alignment Report:

Legend:
Total - total reads considered ( random 10% of total reads sequenced )
Total Unique - total reads aligning to only a single location in the reference
U0 - # of unique hits with 0 mismatches
U1 - # of unique hits with 1 mismatches
U2 - # of unique hits with 2 mismatches
R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches
NM - # of reads without a match to the reference

Mapping Algorithm : GEM allowing up to 2 mismatches

Lane 3

Sample: T0
Index: GCCAAT
Lab ID: 4632
Application: Truseq Stranded mRNA
Reference: M.musculus mRNA Refseq (Release 42)

Total	8710504
Total Unique	5408101	62.1%

U0	4741369	54.4%
U1	597529	6.9%
U2	69203	0.8%
R012	1807292	20.7%
NM	1464845	16.8%

------------------------------------------------

Lane 3

Sample: aT0
Index: CTTGTA
Lab ID: 4633
Application: Truseq Stranded mRNA
Reference: M.musculus mRNA Refseq (Release 42)

Total	8348734
Total Unique	5340303	64.0%

U0	4663144	55.9%
U1	608613	7.3%
U2	68546	0.8%
R012	1702253	20.4%
NM	1278643	15.3%

------------------------------------------------