##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	7304_ACTGAT_R1.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	57277961	
Filtered Sequences	0	
Sequence length	50	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73826379748399	31.0	31.0	34.0	30.0	34.0
2	32.11197462144297	33.0	31.0	34.0	30.0	34.0
3	32.22782729643606	34.0	31.0	34.0	30.0	34.0
4	35.86887775212529	37.0	35.0	37.0	35.0	37.0
5	35.653981013744534	37.0	35.0	37.0	33.0	37.0
6	35.663209991012074	37.0	35.0	37.0	33.0	37.0
7	35.671763333195464	37.0	35.0	37.0	33.0	37.0
8	35.662235829239805	37.0	35.0	37.0	33.0	37.0
9	37.32222772385351	39.0	37.0	39.0	34.0	39.0
10	37.303988247766014	39.0	37.0	39.0	34.0	39.0
11	37.36648815065187	39.0	37.0	39.0	34.0	39.0
12	37.361094261019524	39.0	37.0	39.0	34.0	39.0
13	37.30948374716062	39.0	37.0	39.0	34.0	39.0
14	38.62883907477084	40.0	38.0	41.0	34.0	41.0
15	38.624970972692275	40.0	38.0	41.0	34.0	41.0
16	38.662136558946294	40.0	38.0	41.0	34.0	41.0
17	38.62392156382801	40.0	38.0	41.0	34.0	41.0
18	38.65381258945303	40.0	38.0	41.0	34.0	41.0
19	38.558992908284566	40.0	38.0	41.0	34.0	41.0
20	38.52395882248671	40.0	38.0	41.0	34.0	41.0
21	38.51435680470539	40.0	38.0	41.0	34.0	41.0
22	38.466592237806786	40.0	38.0	41.0	34.0	41.0
23	38.42738570948781	40.0	38.0	41.0	34.0	41.0
24	38.43416631049419	40.0	38.0	41.0	34.0	41.0
25	38.39646053392159	40.0	38.0	41.0	34.0	41.0
26	38.295183273021884	40.0	38.0	41.0	34.0	41.0
27	38.22829204761671	40.0	38.0	41.0	33.0	41.0
28	38.153467369412816	40.0	38.0	41.0	33.0	41.0
29	38.10312750134384	40.0	38.0	41.0	33.0	41.0
30	38.063601967255785	40.0	38.0	41.0	33.0	41.0
31	38.025237089008805	40.0	38.0	41.0	33.0	41.0
32	37.939980318084295	40.0	37.0	41.0	33.0	41.0
33	37.89736129049705	40.0	37.0	41.0	33.0	41.0
34	37.8595588973567	40.0	37.0	41.0	33.0	41.0
35	37.82185142728806	40.0	37.0	41.0	33.0	41.0
36	37.77685022691363	40.0	37.0	41.0	33.0	41.0
37	37.73893517263996	40.0	37.0	41.0	33.0	41.0
38	37.65486915639333	40.0	37.0	41.0	32.0	41.0
39	37.60232077744527	40.0	37.0	41.0	32.0	41.0
40	37.49759824725604	40.0	37.0	41.0	32.0	41.0
41	37.423152528072706	40.0	37.0	41.0	31.0	41.0
42	37.29660017052632	40.0	37.0	41.0	31.0	41.0
43	37.21818582194293	40.0	37.0	41.0	31.0	41.0
44	37.109039845185826	39.0	36.0	41.0	31.0	41.0
45	37.37455521505034	40.0	37.0	41.0	32.0	41.0
46	37.442354049579386	40.0	37.0	41.0	32.0	41.0
47	37.36549249719277	40.0	37.0	41.0	32.0	41.0
48	37.30556141829141	40.0	37.0	41.0	32.0	41.0
49	37.22027198209797	40.0	37.0	41.0	31.0	41.0
50	37.094640607056526	40.0	37.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2335.0
3	262.0
4	313.0
5	558.0
6	769.0
7	1080.0
8	1445.0
9	1840.0
10	2567.0
11	3608.0
12	5117.0
13	7406.0
14	10839.0
15	14767.0
16	19012.0
17	23094.0
18	27809.0
19	33389.0
20	40763.0
21	50659.0
22	62818.0
23	81846.0
24	112959.0
25	158001.0
26	225381.0
27	322253.0
28	453000.0
29	624638.0
30	842742.0
31	1109262.0
32	1432824.0
33	1841994.0
34	2405121.0
35	3200776.0
36	4354489.0
37	6101693.0
38	1.001752E7
39	2.3683012E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.057348105647755	29.83826854226302	22.605940403947134	22.498442948142095
2	19.82383590784595	34.28620303016722	26.418951959550373	19.47100910243645
3	18.77652830734688	34.175460427751986	27.430713008590256	19.617298256310875
4	19.53661548811069	35.18012975357136	27.747787670025474	17.535467088292474
5	17.831887379247895	35.00810283916962	30.852438794136066	16.30757098744642
6	16.84856414494224	33.21342915820624	33.25570894536556	16.68229775148595
7	18.159851197920084	29.594929223233045	33.80389056863916	18.441329010207706
8	18.132098596599786	28.497721086744725	34.6583089666306	18.711871350024882
9	18.63958365388297	26.628930892935166	33.90418796799556	20.827297485186307
10	19.28841880387467	29.309746204129016	30.06459151016217	21.33724348183414
11	20.409022590730842	31.6409849156467	26.49540370335459	21.454588790267863
12	18.921089038068235	33.00418462870911	28.596470115268247	19.478256217954407
13	19.999419321505986	32.021539994632825	29.758819569661366	18.22022111419982
14	18.763283796477452	30.67174059047739	32.05759653551901	18.50737907752614
15	17.38475740940472	30.201172305515268	33.25894386001822	19.155126425061795
16	18.155890709866576	27.973679090985797	33.10998972187575	20.760440477271878
17	18.636738832236016	28.323040340070765	31.574694148068573	21.465526679624645
18	18.406444321577716	29.16556509405075	31.05705176900414	21.370938815367396
19	19.23124847268917	30.25432242603748	28.18138725294359	22.333041848329763
20	19.29187388496598	31.297168556680987	29.192233291963728	20.2187242663893
21	20.546407020319734	30.559567230404728	29.269362434183016	19.62466331509252
22	20.68041667893869	31.426944475205744	29.637889868321253	18.25474897753431
23	19.453768614423968	30.05383344564238	31.92056714449036	18.571830795443294
24	20.017055579613412	30.08105398481685	31.439155667099904	18.462734768469826
25	19.28922706274232	29.79184845563823	31.81795070965619	19.10097377196326
26	18.898058231490385	30.75895765489376	29.71554984418353	20.62743426943232
27	18.69279718796347	29.861107970207033	30.238129386461726	21.20796545536777
28	19.59948230598921	29.90319578805488	30.133632032237468	20.363689873718442
29	21.104250913228086	28.963934420619008	30.151060648234836	19.780754017918078
30	19.4214562704233	29.789738629432904	30.9153739261528	19.873431173990994
31	19.889389135966848	29.597046456891118	30.011279390427564	20.50228501671447
32	19.88302719326108	30.583275109742864	29.64463325615212	19.88906444084394
33	19.375752569125147	30.44598253069798	30.966161662074526	19.212103238102348
34	19.479031385212892	30.913207612261196	29.400217301729718	20.207543700796194
35	18.988130530693994	30.62683219467257	30.312496633740153	20.072540640893273
36	18.78868907362118	30.28902687370453	31.420400946185918	19.501883106488375
37	19.25188642801024	28.661207056584992	31.569662544377238	20.51724397102753
38	19.632318964706165	28.629430436603702	31.6315100671967	20.10674053149343
39	20.314506307233945	28.420215586934038	30.795968802031904	20.469309303800113
40	20.314129335945346	29.08755476105145	30.165448000779914	20.432867902223293
41	21.064623752655244	29.570024184812727	28.999663531437985	20.365688531094044
42	21.01627011478289	30.713451898191696	29.13546800312951	19.134809983895902
43	20.809820349670865	30.130399258922182	29.346595693852333	19.713184697554617
44	20.120729910806418	30.67491202059	29.163469441817096	20.040888626786487
45	18.81283962755757	31.30573255248103	30.77543067709333	19.10599714286807
46	18.327712701784492	30.21642231127289	31.371025381860818	20.084839605081804
47	18.749887139001544	29.779582513675784	31.466339689653122	20.00419065766955
48	18.166373042265814	29.542988152560163	32.35372302479714	19.93691578037689
49	19.7601740332991	28.930489852493185	30.766408997113064	20.542927117094646
50	20.140213988973787	30.226894070311022	29.71481754956248	19.918074391152714
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	47.55579105378985
2	39.2948450102824
3	38.393826563657754
4	37.07208257640316
5	34.13945836669432
6	33.53086189642819
7	36.60118020812779
8	36.843969946624675
9	39.46688113906928
10	40.625662285708806
11	41.863611380998705
12	38.39934525602264
13	38.219640435705806
14	37.2706628740036
15	36.539883834466515
16	38.916331187138454
17	40.102265511860665
18	39.77738313694512
19	41.56429032101893
20	39.51059815135528
21	40.17107033541226
22	38.935165656473
23	38.02559940986726
24	38.47979034808324
25	38.39020083470558
26	39.52549250092271
27	39.90076264333124
28	39.96317217970765
29	40.88500493114616
30	39.29488744441429
31	40.39167415268132
32	39.77209163410502
33	38.58785580722749
34	39.686575086009086
35	39.060671171587266
36	38.29057218010956
37	39.76913039903777
38	39.739059496199594
39	40.78381561103406
40	40.74699723816864
41	41.43031228374929
42	40.15108009867879
43	40.52300504722548
44	40.1616185375929
45	37.91883677042564
46	38.412552306866296
47	38.75407779667109
48	38.1032888226427
49	40.30310115039375
50	40.0582883801265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1805.0
1	2991.5
2	4178.0
3	5680.0
4	7182.0
5	9289.5
6	11397.0
7	15208.0
8	19019.0
9	25180.0
10	31341.0
11	41714.5
12	52088.0
13	67980.0
14	83872.0
15	108162.0
16	132452.0
17	165561.0
18	198670.0
19	243967.0
20	289264.0
21	346899.5
22	404535.0
23	489594.0
24	574653.0
25	717981.0
26	861309.0
27	1177695.0
28	1494081.0
29	2191353.5
30	2888626.0
31	3852954.0
32	4817282.0
33	5978010.0
34	7138738.0
35	7866712.5
36	8594687.0
37	8016316.0
38	7437945.0
39	6412553.0
40	5387161.0
41	4377382.5
42	3367604.0
43	2830819.5
44	2294035.0
45	2070927.0
46	1847819.0
47	1703280.5
48	1558742.0
49	1504770.5
50	1450799.0
51	1362816.0
52	1274833.0
53	1133229.5
54	991626.0
55	883142.5
56	774659.0
57	694019.5
58	613380.0
59	548898.0
60	484416.0
61	434907.5
62	385399.0
63	349669.5
64	313940.0
65	290106.0
66	266272.0
67	244677.0
68	223082.0
69	206045.0
70	189008.0
71	177685.5
72	166363.0
73	157582.5
74	148802.0
75	137333.0
76	125864.0
77	115007.0
78	104150.0
79	94519.0
80	84888.0
81	74094.5
82	63301.0
83	55179.0
84	47057.0
85	39373.5
86	31690.0
87	25748.0
88	19806.0
89	15251.5
90	10697.0
91	8079.0
92	5461.0
93	3937.5
94	2414.0
95	1721.0
96	1028.0
97	719.5
98	411.0
99	270.5
100	130.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05578934627229486
2	0.0
3	0.04441324299236141
4	0.0
5	0.005193969806292511
6	0.0
7	4.6614787841347914E-4
8	3.491744407591604E-6
9	6.983488815183208E-5
10	0.0
11	0.0
12	0.0
13	2.4093036412382068E-4
14	3.491744407591604E-6
15	0.003380008586548673
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0029505240244149054
25	0.00801879103203412
26	0.01568142413449389
27	0.0241628713005339
28	0.019078891443080524
29	0.0145151815023583
30	0.003093685545126161
31	7.507250476321949E-5
32	3.561579295743436E-4
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	4.364680509489505E-5
41	0.0012098894372304908
42	0.0
43	0.009956709178247458
44	0.00756486425904721
45	0.02242049084114569
46	0.016042819680679627
47	0.030132008365311748
48	0.026996421887294487
49	0.025205157006199994
50	0.0023831155581812698
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5.7277961E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	84.28259402231205
#Duplication Level	Relative count
1	100.0
2	90.63909865135467
3	70.02581485681199
4	43.675360081063474
5	25.03076069386475
6	13.686699317233227
7	8.255928972954715
8	5.802311274095877
9	4.849332915148737
10++	120.55538131197375
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT	109471	0.19112237602173024	TruSeq Adapter, Index 5 (97% over 37bp)
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA	71076	0.12408961275699043	No Hit
CGCCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTC	68498	0.11958875421560484	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTC	35427550	7.988691	12.486029	6
GAAAA	31927660	7.3053913	15.199326	1
GTGGA	10704485	5.995404	10.585954	21
TCCAC	11093775	5.874612	9.378337	16
CACAC	10243210	5.41866	7.9987273	31
GCCAT	9646275	5.2044764	15.014776	2
GTCCT	9334195	5.0412498	14.700407	14
GTGTG	8987745	5.039035	12.787477	22
AGGAC	8805410	4.835492	8.084058	41
TTTCT	21307900	4.804798	9.0357485	29
TTTCA	21261400	4.789415	9.419886	8
AGAAA	20652925	4.7256107	7.424045	1
TTCCA	13522340	4.6703243	10.101091	8
CATTT	20707735	4.664694	8.57144	34
TTTCC	13322995	4.606181	8.958838	37
TTCAG	12646990	4.4504094	8.37456	18
CGCCA	5371780	4.4436536	17.052395	1
GAGAA	12319055	4.40778	6.754887	7
AAATG	19209695	4.39988	7.268791	3
TTCTC	12697975	4.390092	10.453415	30
CAGTG	7968125	4.380172	10.877177	20
GGAAA	12209135	4.368451	7.327642	1
TGGAA	11937005	4.27545	7.687846	9
TGAAA	18572825	4.254008	6.5610733	9
TCAGT	12053530	4.2415743	8.080157	19
CACGT	7808890	4.213148	9.165997	11
CACTG	7758020	4.185702	6.3660507	18
CTCGC	5003890	4.143561	7.861109	16
ATGGC	7480525	4.112132	7.551723	35
AGTGG	7296150	4.086452	8.691261	20
CGTCC	4933125	4.084963	11.154582	13
TCGCC	4919830	4.0739536	7.815215	17
GGACG	4722750	4.055573	7.722651	42
CTGAA	11338890	3.9860194	6.579964	19
TCACG	7351005	3.9661045	8.698262	40
ATTTC	17343615	3.906881	8.049282	28
CCAGG	4579030	3.8593407	10.838269	10
ACTGA	10899220	3.8314595	6.0544295	5
CGTGA	6855815	3.768722	7.4723763	26
ACGTG	6793720	3.7345874	6.6877465	26
ATTTT	25331305	3.7217185	6.767906	4
CCACT	6891655	3.649416	6.0757284	16
GAAAT	15888660	3.639214	5.2247515	10
TGGCG	4107475	3.5308247	7.50068	36
CCTGG	4123030	3.4785647	5.7911754	30
CCATA	9996615	3.4490879	9.790372	3
GCGAG	4000645	3.4354787	7.42913	38
AAAAT	23316785	3.415253	6.3193207	2
GGCGA	3973405	3.4120872	7.449866	37
TGAGA	9457330	3.3873107	6.175421	6
TCTCA	9786025	3.3798816	9.500897	31
CTCAT	9505505	3.2829964	9.266908	32
CAGTT	9265065	3.2603278	6.135263	11
GTTTT	14122130	3.244534	5.6227083	13
TTTTT	21945125	3.2275126	5.2741184	45
ATGAG	9006515	3.225843	6.1315923	5
TCATT	13684385	3.0825908	6.6328545	33
CCACC	3795770	3.081799	5.683928	40
ACACA	8930325	3.0780423	4.2222404	32
AAAAA	20507255	3.0006669	4.574237	2
CGTCA	5552915	2.9959767	5.9407964	1
GGTGG	3386040	2.9655886	6.437171	7
AATGA	12833805	2.9395154	5.4174542	4
TCGTC	5364255	2.8971484	5.320366	15
AAAAC	12850355	2.8858511	5.046995	3
AACTG	8203485	2.8838139	5.4315033	46
GGTCC	3340880	2.8186715	9.845212	13
TGTGT	7792925	2.7968862	5.1224575	23
ACGTC	5155240	2.781418	7.2678285	12
TATGG	7651760	2.7434163	5.0735636	34
AAGTG	7304400	2.6162004	5.791823	20
CCATG	4832175	2.6071143	5.137032	39
GACGT	4694485	2.5806134	5.2138324	25
TCCAG	4771255	2.5742462	7.3961477	9
TCCTA	7451665	2.5736442	6.5891533	15
ATTCC	7222705	2.494566	6.6019177	7
AGTGT	6937405	2.4872959	7.553893	21
CATAT	11016145	2.4789984	6.772649	4
TTCAC	7167140	2.4753754	5.564724	39
CAGGT	4097105	2.2522266	7.1310005	11
TGTGC	3647215	2.0069673	6.278832	23
CCGTG	2372950	2.0020373	6.9048486	40
AGGTC	3614685	1.9870347	6.7506013	12
CCTTC	3685680	1.9537157	5.5693436	16
GTGCA	3461980	1.9030912	6.18687	24
TCTCG	2889920	1.5607997	5.023624	43
>>END_MODULE